Reading Room Production — page 79

1) Timeline information "Year 2017: Amplification of full-length RdRp gene and ORF8 gene Year 2018: NGS and gap filling of the genome sequence September 2020: RACE for determination of the 5'end sequence" and "anal swab, not a fecal pellet, they used the High Pure Viral RNA kit for the RNA extraction" "for the 5'race done in September 2020 they used the viral RNA previously extracted from that anal swab sample." could go into the category called "description" for the Biosample accession of RaTG13 sample https:/Awww.ncbi.nim.nih.gov/biosample/?term=SAMN14082201, as in: https://www.ncbi.nlm.nih.gov/biosample/SAMN02928182 2) The information about the "Method of genome sequence assembly" could go into the "Comment" section of the RaTG13 full sequence accession: https://www.ncbi.nim.nih.gov/nuccore/1916859392 3) the list of primers used for amplicons and the points related to the amplicon names could go into the "Strategy" section of the SRA Experiment accession: httos://www.ncbi.nlm.nih.gov/sra/SRX8357956 (see https://www.ncbi.nlm.nih.gov/sra/docs/submitmeta/ for details) From: Clare Thomas Sent: 29 November 2021 09:37 To: Bown. iov.cn Subject: RE: RE: Matters Arising regarding your Nature paper Dear Zhengli, Thank you very much. All the best, Clare From: @wh iov.cn Bown iov.cn> Sent: 29 November 2021 09:07 To: Clare Thomas Bo nature.com> Subject: Re: RE: Matters Arising regarding your Nature paper [External - Use Caution] Dear Clare,