Reading Room Production — page 302
of 496 pages · text recovered by OCR
← p.301 p.303 → · this page in the original PDF · package
1) Timeline information
"Year 2017: Amplification of full-length RdRp gene and ORF8 gene
Year 2018: NGS and gap filling of the genome sequence
September 2020: RACE for determination of the 5'end sequence"
and
"anal swab, not a fecal pellet, they used the High Pure Viral RNA kit for the RNA extraction"
"for the 5'race done in September 2020 they used the viral RNA previously extracted from that anal swab sample."
could go into the category called "description" for the Biosample accession of RaTG13 sample
https:/Awww.ncbi.nim.nih.gov/biosample/?term=SAMN14082201, as in:
https://www.ncbi.nlm.nih.gov/biosample/SAMN02928182
2) The information about the "Method of genome sequence assembly" could go into the "Comment" section of the RaTG13
full sequence accession:
https://www.ncbi.nim.nih.gov/nuccore/1916859392
3) the list of primers used for amplicons and the points related to the amplicon names could go into the "Strategy" section
of the SRA Experiment accession:
httos://www.ncbi.nlm.nih.gov/sra/SRX8357956 (see https://www.ncbi.nlm.nih.gov/sra/docs/submitmeta/ for details)
From: Clare Thomas
Sent: 29 November 2021 09:37
To: Bown. iov.cn
Subject: RE: RE: Matters Arising regarding your Nature paper
Dear Zhengli,
Thank you very much.
All the best,
Clare
From:
@wh iov.cn Bown iov.cn>
Sent: 29 November 2021 09:07
To: Clare Thomas Bo
nature.com>
Subject: Re: RE: Matters Arising regarding your Nature paper
[External - Use Caution]
Dear Clare,
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Records on this page
| Record | Date | Type | Pages |
|---|---|---|---|
| reading_room:email:00299 | — | 300–302 | |
| reading_room:email:00301 | 2021-11-29 | 302–303 | |
| RE: RE: Matters Arising regarding your Nature paper | 2021-11-29 | 302 |