Reading Room Production — page 251

>E2 Expressing Mice with on experiments as described this occurred? ters of 35a, red ained te] > ne for _--_i > Finally, in the RPPR (6/1/2017-5/31/2018) on page 28 under "In Vivo Infection of Human ACE2 Expressing Mice with SARSr-Cov S Protein variants", it appears to be showing the results from their gain of function experiments as described in the SF 424. This is more of less the same as the question above, but do you know where this occurred? These are not the results of GoF experiments. The figure you reference (Fig 35) shows weight loss and lung viral titers of chimeric viruses with bat CoV (wild type WIV-1, SHC014, WIV16, and 4231) spike proteins expressed on the WIV-1 backbone. Weight loss and viral titers were comparable across all chimeras when compared to the wild type (Fig 35a, red series; Fig 35b small box pattern). There are no statistical differences reported, so the chimeric viruses have not gained any function/attribute they did not already exhibit. From: Sanders, Ashley (NIH/OD) [E] @nih.gov> Sent: Thursday, May 21, 2020 3:04 PM To: Stemmy, Erik (NIH/NIAID) [E] @nih.gov>; Linde, Emily (NIH/NIAID) [E] @mail.nih.gov> Cc: Shannon, Mike (NIH/OD) [E] @nih.gov> Subject: RE: Grant Questions - FBI Inquiry - 1-R01AI110964-01 - 2-R01AI110964-06 Hi Erik, Thank you for providing this information. We would like to set up a call with the agent as they may need additional context or have other questions. Would you be available next Tuesday around 11:30AM? I'll set up a conference line for us and send an invite. If that time doesn't work for you, please let me know alternative times you have available. Thank you, Ashley From: Stemmy, Erik (NIH/NIAID) [E] @nih.gov> Sent: Thursday, May 21, 2020 11:22 AM To: Linde, Emily (NIH/NIAID) [E] @mail.nih.gov>; Sanders, Ashley (NIH/OD) [E] @nih.gov> Cc: Shannon, Mike (NIH/OD) [E] @nih.gov> Subject: RE: Grant Questions - FBI Inquiry - 1-R01AI110964-01 - 2-R01AI110964-06 Hi Ashley, I would be happy to answer questions related to this award. Would it be best if I just addressed the comments at the end of this email thread? I'll say generally that NIAID has an extensive review process for P3CO (and formerly for GoF) and that all of this work was formally evaluated by the respective committees and determined not to be gain-of-function, nor subject to P3CO oversight. We also continually monitored the progress (until the award was terminated) for evidence of unexpected increases in viral replication. Erik Erik J. Stemmy, Ph.D. Program Officer Respiratory Diseases Branch Division of Microbiology and Infectious Diseases NIAID/NIH/HHS 5601 Fishers Lane, Room 8E18 Bethesda, MD 20892-9825 Phone: Email: @nih.gov