Reading Room Production — page 13

let us know if you are able to 2rtain aspects were fficial to sign off on 4/27/20. you understand, since this is a sensitive I'm happy to discuss further if you have ite dn't cates hen it at Good afternoon, We are following up on a time-sensitive request for information from the FBI. Please let us know if you are able to accommodate their request or if you can recommend a POC to address the concerns. Thank you, Ashley From: Sanders, Ashley (NIH/OD) [E] Sent: Wednesday, May 13, 2020 11:59 AM To: Linde, Emily (NIH/NIAID) [E] @mail.nih.gov> Cc: Shannon, Mike (NIH/OD) [E] @nih.gov> Subject: Grant Questions - FBI Inquiry - 1-R01AI110964-01 - 2-R01AI110964-06 Importance: High Good afternoon Emily, OMA/DPI received questions related to an FBI inquiry for the following NIAID grants: 1R01AI110964-01 through 2R01AI110964-06. The agent is requesting clarification on the details of the study, where certain aspects were performed, and whether seamless cloning techniques were used. I note you were the last official to sign off on 4/27/20. Would you be able to provide a response to the below-captured questions and/or be available for a joint call with our office and the agent? If not, who would be the best SME on this matter? I'm sure you understand, since this is a sensitive matter, we need to maintain confidentiality and limit the touch-points within NIH. I'm happy to discuss further if you have any questions. Thank you, Ashley Ashley M. Sanders, MPS Senior Program Investigations Officer NIH, OMA, Division Program Integrity 6011 Executive Blvd. Rockville, Maryland 20852 Office: @nih.gov FBI Questions: SF 424 AI110964-06 (received date 11/05/2018) Both SF 424s seem to be describing "gain of function" experiments. On page 192 of SF 424 AI110964-06 (received date 11/05/2018) under P3CO Research, it indicates they are conducting gain of function of SARSr-CoV. Were they also conducting Seemless Cloining and Assembly? From a review of their experimental details, it looks as if they were generating recombinant DNA of the viruses using WIV1 as the backbone but I could not determine if they were using Seemless Cloning techniques. I could not determine exactly where they were conducting these gain of function experiments. I know UNC-Chapel Hill has done these but within the document, I could not determine where this was occurring. Do you know where this was occurring? It then appears that the recombinant DNA of the virus was then injected into humanized mice. However, again I couldn't determine where this occurred or was to occur. On page 187 of the same SF 424 under "Vertebrate Animals" it indicates that work with vertebrate animals will be conducted at Wuhan University at the School of Medicine and UNC - CH. Then under "Laboratory Mice" it states that lab mice will be sourced commercially by Wuhan Center for Animal Experiment at