Attachment

Fig.10 Cas-OFFinder 76 OTSs in mouse genome NGS for top 5 OTS (gDNA, week 16) A A5A8A9 A10 A11 A8 A11 A4 A7A8 A10 A4A5 A7A8 A10 A4 A8A9 A13 0.00 0.05 0.10 0.15 0.20 % of reads containing A>G conversions non-transduced HDAd-EF1α.ABE8e mOTS1' mOTS2' mOTS3' mOTS4' mOTS5' 0.00 0.05 0.10 0.15 0.20 0.5 1.0 1.5 % of reads containing indels mOTS1' mOTS2' Cas-OFFinder 89 OTSs in human genome NGS for top 5 OTS (gDNA from Thal cells, day 18 ED) B mOTS3' mOTS4' mOTS4' A5 A8 A9 A11 A5 A8 A9 A11 A5 A8 A9 A11 A5 A9 A11 A12 A5 A8 A11 0.00 0.05 0.10 0.15 0.20 % of reads containing A>G conversions non-transduced HDAd-EF1α.ABE8e hOTS1 hOTS2 hOTS3 hOTS4 hOTS5 0.00 0.05 0.10 0.15 0.20 0.5 1.0 1.5 % of reads containing indels hOTS1 hOTS2 hOTS3 hOTS4 hOTS5